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Chain conformations of amphiphilic polymer molecules in aqueous single-chain emulsions have been directly observed using AFM. These observations are made possible by the high lateral resolution of the AFM tip and by using polymerase chain reaction (PCR)-generated deoxyribonucleic acid (DNA) with selectively modified double-stranded bases that distinguish denatured and single-stranded DNAs, as well as by using double-stranded DNA with non-complementary bases to act as a control. The denatured DNA is created by creating a double-stranded structure in the parent DNA. Double-stranded structures are then selectively broken at specific sites using DNA primers. Upon injection of DNA solution into an aqueous single-chain emulsion in the AFM, we observe that only those molecules that are threaded at the single-chain emulsion interface remain in solution. This observation is consistent with theory, which suggests that molecules with surface adsorption energies that match the interfacial free energy of the emulsion are able to remain in solution, whereas molecules with weakly adsorbed states are ejected.Visual postural control after bilateral subthalamic nucleus stimulation: a controlled case study.
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