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Previous phylogenetic analysis of schistosomes and cestodes based on mitochondrial DNA sequences have been performed using methods requiring substantial amounts of DNA and long-term sequencing. In order to assess the ability of rapid DNA-based methods to determine the phylogeny of schistosomes, the sequence-tagged sites (STSs) and PCR-based restriction fragment length polymorphism (RFLP) methods were applied. The results revealed that analysis of STSs and PCR-RFLP reveals six different types of schistosomes and the extent of changes for individual loci was not uniform. Moreover, when strains sharing the same phenotype were compared it was found that the sequence variations for individual loci are not exactly coincident. These results indicate that the rapid molecular methods could be used for estimation of phylogeny of schistosomes, but may not sufficiently represent the actual sequence of the organisms./*
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